"Temperature dependence of the primary electron transfer reaction in pigment-modified bacterial reaction centers"H. Huber, M. Meyer, H. Scheer, W. Zinth, J. Wachtveitl
Photosynthesis Research (1998) 55, 153-162
The initial electron transfer steps in pigment modified reaction centers, where bacteriopheophytin is replaced by plant pheophytin (R26.Phe-a RCs)have been investigated over a wide temperature range by femtosecond time-resolved spectroscopy. The experimental data obtained in the maximum ofthe bacteriochlorophyll anion band at 1020 nm show the existence of a high and long-lived population of the primary acceptor P+BA- even at 10 K. Thedata suggest a stepwise electron transfer mechanism with BA as primary acceptor also in the low temperature domain. A detailed data analysissuggests that the pigment modification leads to a situation with almost isoenergetic primary and secondary acceptor levels, approximately 450 cm-1below P*. A Gaussian distribution (with sigma = 400 cm-1) of the delta-G values has to be assumed to account for the strong dispersive character ofthe kinetics in this sample. Based on these assumptions, a model is presented that reproduces the observed kinetics, heterogeneity and temperature dependence.
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